Open-Volume Microfluidics for Bioprinting of In Vitro Tumor Models

The microenvironment of cancer cells has been demonstrated to have a profound influence upon both, tumor progression, as well on their response drugs. Control over the is an essential element in generation physiologically relevant vitro models. This would enable natural cell-to-cell interaction between various cell types, which increasingly important aspect within research. In order microenvironments at cellular level, we utilize open-volume microfluidics for high-precision patterning mammalian and generate confluent biological tissues. Within this technique, individual are circulated confined fluid volume close surface, they adhere. surface can be pre-coated substrate, employing extracellular matrix components, optimized efficient attachment. Additionally, layer patterned. To demonstrate our approach modelling, simple skin liver models were constructed using A431 HaCaT cells, Hep G2 3T3-J2 respectively. functionality model was probed by measuring albumin production three weeks, indicating suitability prolonged studies. As further demonstration, co-printed neuroblastoma (SH-SY5Y) human mesenchymal stem (MSCs) studying cells. presented microfluidic bioprinting technique great potential building multicellular advance understanding disease mechanisms screening drug compounds.